For cells, cell lines and tissues in culture till half confluency.There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.Low range, broad range, wide range or standard range protein kDa markers or DNA bp-kb ladders are available from Trevigen. ELISA kits in the standard range can be adapted to the high sensitive range only by adding some points to the lower end of the standard curve.