Cultrex Stem Cell Qualified Human Fibronectin, PathClear

-80° C

Saturday, July 28, 2007

Cultrexes are the best 3D matrixes for cell cultures known as matrigel or cultrex. Basal membrane extracts higher the differentiation capacity or from basal membrane over what can be achieved by 2D monolayer cultures from epithelial cells. You can use cultrex for high differentiation in a 3D matrix as Basement Membrane Extracts (BME), extracellular matrix from lumen epithelial or endothelial cells. BME, collagen I, collagen IV, laminin I, fibronectin and vitronectin are pathogen tested and suitable for 3d and pluripotent stem cell culture. Cultrex Basement Membrane and the PathClear Protocol is supplied in a liquid form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor at a concentration of 13 - 17 mg/ml from Murine Engelbreth-Holm-Swarm (EHS) tumor with Dulbecco’s Modified Eagle’s medium without phenol red and with 11 ug/ml gentamicin sulfate with cultrex BME for thin Layer Method (non-gelling).

Cultrex 3D cell culture for cancer studies cell invasion and 3 dimensional cell expansion up to 2/3 conflunecy

cell, stem

For cells, cell lines and tissues in culture till half confluency.

The gelatin protein mixture of Trevigen is called Cultrex and it is a matrigel like secreted supernatant of the EHS mouse sarcoma cell line. It is a 3D structure similar to Tissues and cells will to adhere to this basement membrane extract BME very well. Cultrexes are the best medium for 3D cell culture studies. They can be used as a metastasis model or an iPSC model (induced pluripotent stem cell). The cultrex medium is useful for cell migration studies using the Pathclear endotoxin free and sterile basement membrane extract. Enghelbreth Holm Swarm sarcoma cells are sold by Corning. A collagen, laminin, fibronectin and vitrobectin Cultrexes are available from Trevigen. Cultrex® Human has been tested for the viruses should be handled in a BSL2 ( biosafety level 2 ) environment.Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.