The gelatin protein mixture of Trevigen is called Cultrex and it is a matrigel like secreted supernatant of the EHS mouse sarcoma cell line. It is a 3D structure similar to Tissues and cells will to adhere to this basement membrane extract BME very well. Cultrexes are the best medium for 3D cell culture studies. They can be used as a metastasis model or an iPSC model (induced pluripotent stem cell). The cultrex medium is useful for cell migration studies using the Pathclear endotoxin free and sterile basement membrane extract. Enghelbreth Holm Swarm sarcoma cells are sold by Corning. A collagen, laminin, fibronectin and vitrobectin Cultrexes are available from Trevigen. Cultrex® Human has been tested for the viruses should be handled in a BSL2 ( biosafety level 2 ) environment.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
